Anti-heavy chain Myosin/Myh3 Antibody Picoband™

Product Name

Anti-heavy chain Myosin/Myh3 Antibody Picoband™

View all Myosin Heavy Chain 3 Antibodies

SKU/Catalog Number

A02697-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-heavy chain Myosin/Myh3 Antibody Picoband™ catalog # A02697-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-heavy chain Myosin/Myh3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02697-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat heavy chain Myosin/Myh3 recombinant protein (Position: K44-S119).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02697-1 is reactive to MYH3 in Human, Mouse, Rat

Applications

A02697-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

224 kDa

Calculated molecular weight

223.905kDa

Background of Myosin Heavy Chain 3

Myosin-3 is a protein that in humans is encoded by the MYH3 gene. Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells,Human, Rat
Direct ELISA, 0.1-0.5 μg/ml, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-heavy chain Myosin/Myh3 antigen affinity purified polyclonal antibody (Catalog # A02697-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for heavy chain Myosin/Myh3 at approximately 224 kDa. The expected band size for heavy chain Myosin/Myh3 is at 224 kDa.

Click image to see more details

Figure 2. IHC analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
heavy chain Myosin/Myh3 was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 3. IHC analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
heavy chain Myosin/Myh3 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. Flow Cytometry analysis of U20S cells using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
Overlay histogram showing U20S cells stained with A02697-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MYH3 (Source: Uniprot.org, NCBI)

Alternative Names

HEMHC; Muscle embryonic myosin heavy chain; MYHC-EMB; MYHSE1; Myosin heavy chain, fast skeletal muscle, embryonic; myosin, heavy chain 3, skeletal muscle, embryonic; myosin, heavy polypeptide 3, skeletal muscle, embryonic; myosin, skeletal, heavy chain, embryonic 1; myosin-3; SMHCEMyosin heavy chain 3 MYH3 CPSFS1A, CPSFS1B, CPSKF1A, CPSKF1B, DA2A, DA2B, DA2B3, DA8, HEMHC, MYHC-EMB, MYHSE1, SMHCE myosin heavy chain 3 myosin-3|myosin heavy chain, fast skeletal muscle, embryonic|myosin, heavy chain 3, skeletal muscle, embryonic|myosin, heavy polypeptide 3, skeletal muscle, embryonic|myosin, skeletal, heavy chain, embryonic 1

For more info on MYH3, check out the MYH3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MYH3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis